Composite
Part:BBa_K4759203:Design
Designed by: Jianghua Chen Group: iGEM23_Jiangnan-China (2023-10-07)
T7-RBS4-OleP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 102
Illegal AgeI site found at 200 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Olep is selected to synthesize the above gene by adding histidine tags at the C-terminus through E. coli codon optimization,
Source
T7-RBS4 is from pETDuet, oleP is from Streptomyces