Composite

Part:BBa_K4759203:Design

Designed by: Jianghua Chen   Group: iGEM23_Jiangnan-China   (2023-10-07)


T7-RBS4-OleP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 102
    Illegal AgeI site found at 200
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Olep is selected to synthesize the above gene by adding histidine tags at the C-terminus through E. coli codon optimization,


Source

T7-RBS4 is from pETDuet, oleP is from Streptomyces

References